Identification of differentially expressed genes from limited amounts of RNA

Identification of differentially expressed genes using minute amounts of RNA.

Identification of differentially expressed genes is critical to understand the molecular mechanisms underlying both normal and pathophysiological events. A significant limiting factor associated with such studies can often be the quantity of available starting material (i.e., RNA). Among the existing subtraction hybridization techniques that have been previously used for such studies at the lev...

identification of differentially expressed genes in l02 human hepatocyte induced by hepatitis c virus core protein

Identification of Differentially Expressed Genes in RNA-seq Data of Arabidopsis thaliana: A Compound Distribution Approach

Gene expression is the process by which information from a gene is used in the synthesis of a functional gene product, which may be proteins. A gene is declared differentially expressed if an observed difference or change in read counts or expression levels between two experimental conditions is statistically significant. To identify differentially expressed genes between two conditions, it is ...

Identification of differentially expressed genes in the development of osteosarcoma using RNA-seq

OBJECTIVE Osteosarcoma (OS) is a malignant bone tumor with high morbidity in young adults and adolescents. This study aimed to discover potential early diagnosis biomarkers in OS. RESULTS In total, 111 differentially expressed genes (DEGs) were identified in primary OS compared with normal controls and 235 DEGs were identified in metastatic OS compared with primary OS. AURKB and PPP2R2B were ...

Identification of differentially expressed genes through RNA sequencing in goats (Capra hircus) at different postnatal stages

Intramuscular fat (IMF) content and fatty acid composition of longissimus dorsi muscle (LM) change with growth, which partially determines the flavor and nutritional value of goat (Capra hircus) meat. However, unlike cattle, little information is available on the transcriptome-wide changes during different postnatal stages in small ruminants, especially goats. In this study, the sequencing read...